mouse insulin growth factor ii  (R&D Systems)

Journal: Annals of Translational Medicine

Article Title: Advanced platelet-rich fibrin promotes the paracrine function and proliferation of adipose-derived stem cells and contributes to micro-autologous fat transplantation by modulating HIF-1α and VEGF

doi: 10.21037/atm-21-6812

Figure Lengend Snippet: APRF promotes the paracrine function of ASCs. (A-R) ASCs were treated with APRF at the indicated dose. (A-F) The levels of HIF-1α, HSP70, IGF-2, IL-6, IL-8, and VEGF in the culture medium of ASCs were analyzed by ELISA assays at the indicated dose. (G-L) The mRNA expression levels of HIF-1α, HSP70, IGF-2, IL-6, IL-8, and VEGF in ASCs were measured by qRT-PCR assays. (M-R) The protein expression levels of HIF-1α, HSP70, IGF-2, IL-6, IL-8, and VEGF in ASCs were measured by Western blot analysis. The results of Western blot analysis were quantified by ImageJ software. Data are presented as mean ± SD. Significant differences are indicated by *, P<0.05; **, P<0.01. APRF, advanced platelet-rich fibrin; ASCs, adipose-derived stem cells; HIF-1α, hypoxia-inducible factor-1α; VEGF, vascular endothelial growth factor; qRT-PCR, quantitative reverse transcription-PCR.

Article Snippet: The levels of cytokines in the culture medium were analyzed by ELISA assays, including the HIF-1α ELISA Kit (Elabscience, Shanghai, China), heat shock protein 70 ( HSP70 ) ELISA Kit (Elabscience, Shanghai, China), Human IGF-2 ELISA Kit (Boster, Wuhan, Hubei, China), Human interleukin-6 ( IL-6 ) ELISA Kit (Elabscience, Shanghai, China), Human interleukin-8 ( IL-8 ) ELISA Kit (Elabscience, Shanghai, China), and Human VEGF ELISA Kit (Mlbio, Shanghai, China).

Techniques: Enzyme-linked Immunosorbent Assay, Expressing, Quantitative RT-PCR, Western Blot, Software, Derivative Assay, Reverse Transcription

Journal: Annals of Translational Medicine

Article Title: Advanced platelet-rich fibrin promotes the paracrine function and proliferation of adipose-derived stem cells and contributes to micro-autologous fat transplantation by modulating HIF-1α and VEGF

doi: 10.21037/atm-21-6812

Figure Lengend Snippet: APRF regulates the paracrine function of ASCs by modulating HIF-1α and VEGF. (A,B) ASCs were treated with lentivirus vectors carrying the shRNA of HIF-1α and VEGF. The efficiency of shRNAs of HIF-1α and VEGF was validated by qRT-PCR assays in ASCs. (C-T) ASCs were treated with APRF, or co-treated with APRF and the shRNA of HIF-1α or VEGF. (C-H) The levels of HIF-1α, HSP70, IGF-2, IL-6, IL-8, and VEGF in the culture medium of ASCs were analyzed by ELISA assays at the indicated dose. (I-N) The mRNA expression levels of HIF-1α, HSP70, IGF-2, IL-6, IL-8, and VEGF in ASCs were measured by qRT-PCR assays. (O-T) The protein expression levels of HIF-1α, HSP70, IGF-2, IL-6, IL-8, and VEGF in ASCs were measured by Western blot analysis. The results of Western blot analysis were quantified by ImageJ software. Data are presented as mean ± SD. Significant differences are indicated by *, P<0.05; **, P<0.01; ##, P<0.01. APRF, advanced platelet-rich fibrin; ASCs, adipose-derived stem cells; HIF-1α, hypoxia-inducible factor-1α; VEGF, vascular endothelial growth factor; qRT-PCR, quantitative reverse transcription-PCR.

Article Snippet: The levels of cytokines in the culture medium were analyzed by ELISA assays, including the HIF-1α ELISA Kit (Elabscience, Shanghai, China), heat shock protein 70 ( HSP70 ) ELISA Kit (Elabscience, Shanghai, China), Human IGF-2 ELISA Kit (Boster, Wuhan, Hubei, China), Human interleukin-6 ( IL-6 ) ELISA Kit (Elabscience, Shanghai, China), Human interleukin-8 ( IL-8 ) ELISA Kit (Elabscience, Shanghai, China), and Human VEGF ELISA Kit (Mlbio, Shanghai, China).

Techniques: shRNA, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Expressing, Western Blot, Software, Derivative Assay, Reverse Transcription

Journal: Annals of Translational Medicine

Article Title: Advanced platelet-rich fibrin promotes the paracrine function and proliferation of adipose-derived stem cells and contributes to micro-autologous fat transplantation by modulating HIF-1α and VEGF

doi: 10.21037/atm-21-6812

Figure Lengend Snippet: APRF contributes to micro-autologous fat transplantation in vivo. (A-P) BALB/c mice (n=3) were transplanted with fat, co-transplanted with fat and ASCs or APRF, or co-transplanted with fat, ASCs, and APRF as indicated. (A) Representative images of transplanted fat in mice. (B) The fat retention rate was calculated. (C-H) The serum levels of HIF-1α, HSP70, IGF-2, IL-6, IL-8, and VEGF were analyzed by ELISA assays. (I-N) The protein expression levels of HIF-1α, HSP70, IGF-2, IL-6, IL-8, and VEGF in the fat tissues of the mice were measured by Western blot analysis. The results of Western blot analysis were quantified by ImageJ software. (O) Lipid accumulation was measured by Oil Red O staining. (P) The expression of CD34 was assessed by immunohistochemical staining. Data are presented as mean ± SD. Significant differences are indicated by **, P<0.01. APRF, advanced platelet-rich fibrin; ASCs, adipose-derived stem cells; HIF-1α, hypoxia-inducible factor-1α; VEGF, vascular endothelial growth factor.

Article Snippet: The levels of cytokines in the culture medium were analyzed by ELISA assays, including the HIF-1α ELISA Kit (Elabscience, Shanghai, China), heat shock protein 70 ( HSP70 ) ELISA Kit (Elabscience, Shanghai, China), Human IGF-2 ELISA Kit (Boster, Wuhan, Hubei, China), Human interleukin-6 ( IL-6 ) ELISA Kit (Elabscience, Shanghai, China), Human interleukin-8 ( IL-8 ) ELISA Kit (Elabscience, Shanghai, China), and Human VEGF ELISA Kit (Mlbio, Shanghai, China).

Techniques: Transplantation Assay, In Vivo, Enzyme-linked Immunosorbent Assay, Expressing, Western Blot, Software, Staining, Immunohistochemical staining, Derivative Assay

Journal: Annals of Translational Medicine

Article Title: Advanced platelet-rich fibrin promotes the paracrine function and proliferation of adipose-derived stem cells and contributes to micro-autologous fat transplantation by modulating HIF-1α and VEGF

doi: 10.21037/atm-21-6812

Figure Lengend Snippet: APRF promotes micro-autologous fat transplantation by modulating HIF-1α and VEGF in vivo. (A-P) BALB/c mice (n=3) were co-transplanted with fat, ASCs, APRF, and control shRNA, HIF-1α shRNA, or VEGF shRNA. (A,B) Representative images of transplanted fat in the mice. (B) The fat retention rate was calculated. (C-H) The serum levels of HIF-1α, HSP70, IGF-2, IL-6, IL-8, and VEGF were analyzed by ELISA assays. (I-N) The protein expression levels of HIF-1α, HSP70, IGF-2, IL-6, IL-8, and VEGF in the fat tissues of the mice were measured by Western blot analysis. The results of Western blot analysis were quantified by ImageJ software. (O) Lipid accumulation was measured by Oil Red O staining. (P) The expression of CD34 was assessed by immunohistochemical staining. Data are presented as mean ± SD. Significant differences are indicated by *, P<0.05; **, P<0.01. APRF, advanced platelet-rich fibrin; HIF-1α, hypoxia-inducible factor-1α; VEGF, vascular endothelial growth factor.

Article Snippet: The levels of cytokines in the culture medium were analyzed by ELISA assays, including the HIF-1α ELISA Kit (Elabscience, Shanghai, China), heat shock protein 70 ( HSP70 ) ELISA Kit (Elabscience, Shanghai, China), Human IGF-2 ELISA Kit (Boster, Wuhan, Hubei, China), Human interleukin-6 ( IL-6 ) ELISA Kit (Elabscience, Shanghai, China), Human interleukin-8 ( IL-8 ) ELISA Kit (Elabscience, Shanghai, China), and Human VEGF ELISA Kit (Mlbio, Shanghai, China).

Techniques: Transplantation Assay, In Vivo, Control, shRNA, Enzyme-linked Immunosorbent Assay, Expressing, Western Blot, Software, Staining, Immunohistochemical staining